Metabolomic analyses provide insights into the preharvest rind disorder in Satsuma Owari Mandarin

Citrus fruit’s appearance is the primary criterion used to assess its quality for fresh market, hence rind’s condition a crucial trait. Pre-harvest rind disorder one of major physiological problems in mandarins. The occurs right before harvest following rain events some Mandarin varieties. Despite economic damage caused by this kind disorder, very limited information available about molecular mechanisms underlying occurrence disorder. In present study, we evaluated metabolites, antioxidants, and hormones associated with pre-harvest Mandarins. study was carried out using ten-year-old ‘Owari’ Satsuma mandarin trees grafted on ‘Carrizo’ rootstock grown commercial orchard San Joaquin Valley, California, USA. Samples were collected from healthy tissue fruit (HF_HT), damaged (DF_HT), (DF_DT). Damaged (DF_HT DF_DT) showed lower cellulose concentrations than tissues however, had similar contents pectin hemicellulose. antioxidant activities no significant difference all paired comparisons between samples as expressed malondialdehyde (MDA) content. However, DF_DT higher H 2 O content compared HF_HT, but DF_HT that HF_HT. Furthermore, peroxidase (POD) polyphenol oxidase (PPO) increased HF_HT ( P = 0.0294) 0.0044), respectively. Targeted metabolomics analysis revealed total 76 metabolites identified tissues, relative 43 significantly different across studied samples. hormonal involvement jasmonate O-methyltransferase, jasmonic acid-amido synthetase JAR1-like, JA-isoleucine may key role causing addition, have level acid (JA), 12-oxo-phytodienoic acid, undamaged tissue.